Journal: bioRxiv

Article Title: Nutritionally responsive PMv DAT neurons are dynamically regulated during pubertal transition

doi: 10.1101/2025.02.03.636271

Figure Lengend Snippet: A. Representative low-magnification fluorescent image of bilateral injections of adenoassociated virus (AAV) expressing Cre dependent hM3Dq-mCherry targeted to the PMv of a female DAT Cre mouse. B. High magnification image showing Fos immunoreactivity (Fos-ir) in one PMv side following an intraperitoneal injection of clozapine-N-oxide (CNO) and corresponding fluorescent image of the PMv showing mCherry immunofluorescence. C. High magnification image showing the lack of Fos-ir in one PMv side of a missed AAV injection, following an intraperitoneal injection of clozapine-N-oxide (CNO) and corresponding fluorescent image of the PMv showing the lack of mCherry immunofluorescence. D. Representative estrous cycles of two females with AAV injections centered in the PMv before treatment (drinking water), during the treatment with DMSO (vehicle) and CNO in drinking water. E/M: estrus/metestrus; P: Proestrus; D: Diestrus. E-G. Graphs showing the number of days spent in estrus/metestrus, diestrus and the cycle length (number of days) in the DAT Cre females with bilateral PMv AAV-hM3Dq injections during the DMSO and the CNO treatment.

Article Snippet: Adult virgin DAT-Cre females (n=14) received bilateral injection (50 nL/side) of an adenoassociated virus (AAV) expressing a Cre-dependent hM3Dq-mCherry fusion protein,pAAV8-hSyn-DIO-hM3D(Gq)-mCherry (AAV-hM3Dq, Addgene plasmid # 44361, from Bryan Roth) ( ) in the PMv using the following coordinates: Anteroposterior = -5.4 mm (from rostral rhinal vein); mediolateral = -0.52 mm (from sagittal sinus); dorsoventral = -5.4 mm (from dura mater).

Techniques: Virus, Expressing, Injection, Immunofluorescence